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healthy cd4 cd25 tregs  (MedChemExpress)
97
MedChemExpress healthy cd4 cd25 tregs
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Healthy Cd4 Cd25 Tregs, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/AZD3965/pmc13487165-408-3-21
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healthy cd4 cd25 tregs - by Bioz Stars, 2026-09
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microbeads  (Miltenyi Biotec)
93
Miltenyi Biotec microbeads
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/MACSxpress+Whole+Blood+Treg+Isolation+Kit%2C+human/pm42304385-98-9-13
Average 93 stars, based on 1 article reviews
microbeads - by Bioz Stars, 2026-09
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treg derived lgals9  (Galectin Therapeutics)
86
Galectin Therapeutics treg derived lgals9
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Treg Derived Lgals9, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/lgals3/pm42296961-189-35-37
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tregs  (Galectin Therapeutics)
86
Galectin Therapeutics tregs
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Tregs, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/us12649794-660-8-3
Average 86 stars, based on 1 article reviews
tregs - by Bioz Stars, 2026-09
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treg activity with ipilimumab  (Agenus Inc)
86
Agenus Inc treg activity with ipilimumab
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Treg Activity With Ipilimumab, supplied by Agenus Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/anti+h3k4me2+immunostaining+with/pmc13246693-159-1-5
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treg activity with ipilimumab - by Bioz Stars, 2026-09
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cd8 t cell treg interactions  (Vectra Laboratories)
86
Vectra Laboratories cd8 t cell treg interactions
a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
Cd8 T Cell Treg Interactions, supplied by Vectra Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/cd8+cell+interactions+t+treg/pm42114663-445-8-0
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cd8 t cell treg interactions - by Bioz Stars, 2026-09
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dose anti cd3 cd28 beads  (Miltenyi Biotec)
97
Miltenyi Biotec dose anti cd3 cd28 beads
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Dose Anti Cd3 Cd28 Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/Treg+Expansion+Kit%2C+mouse/pmc13353084-91-20-27
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dose anti cd3 cd28 beads - by Bioz Stars, 2026-09
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france regulatory t cell treg therapy  (Inserm Transfert)
86
Inserm Transfert france regulatory t cell treg therapy
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
France Regulatory T Cell Treg Therapy, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/872+cell+effect+protective+stem+therapy/pm42079605-12-53-40
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france regulatory t cell treg therapy - by Bioz Stars, 2026-09
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tregs  (Abbott Laboratories)
86
Abbott Laboratories tregs
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Tregs, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/tregs/pm42071213-212-5-14
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tregs - by Bioz Stars, 2026-09
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human treg isolation kit  (Miltenyi Biotec)
96
Miltenyi Biotec human treg isolation kit
A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, <t>CD3;</t> white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a <t>3-day-treatment</t> <t>with</t> <t>anti-CD3/CD28</t> Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.
Human Treg Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/treg/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+human/pm41981294-250-1-5
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human treg isolation kit - by Bioz Stars, 2026-09
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Image Search Results


a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs (CD4+CD25+) of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .

Journal: Nature Communications

Article Title: Nrf2-mediated metabolic reprogramming drives regulatory T cell accumulation in hepatocellular carcinoma

doi: 10.1038/s41467-026-73485-3

Figure Lengend Snippet: a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs (CD4+CD25+) of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .

Article Snippet: Freshly isolated human healthy CD4+CD25+ Tregs were pre-incubated in the presence or absence of 100 nM of the MCT1 inhibitor AZD3965 (MedChemExpress; HY-12750) for 2 h and then cultured with low dose IL-2 (50IU/ml) and 1:5 aCD3/CD28 beads in RPMI standard or RPMI high lactate and low glucose (as described above) for 30 min.

Techniques: Fluorescence, Isolation, Transformation Assay

A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, CD3; white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a 3-day-treatment with anti-CD3/CD28 Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.

Journal: Cancer research

Article Title: Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic Cancer

doi: 10.1158/0008-5472.CAN-25-4223

Figure Lengend Snippet: A, Representative confocal images of Tregs in human PDAC and spleen specimens collected from an identical patient. The dotted lines outline cancer ducts. Red, CD3; white, Foxp3; green, αvβ5 integrin; blue, DAPI. Arrowheads, αvβ5 integrin + Tregs. The boxed area is magnified. Scale bars, 20 μm (large panels); 10 μm (small panels). The bar diagram summarizes the average number of αvβ5 + Tregs in the PDAC and spleen from 5 patients. The cells were counted in 5 randomly chosen HPFs per section. B, Flow cytometry analysis of naïve human CD4 + T cells before (left) and after (right) a 3-day-treatment with anti-CD3/CD28 Abs and TGF-β1. Note the induction of CD25 + Foxp3 + iTregs (red) that express αvβ5 integrin. CD25 − Foxp3 − non-Tregs (blue) remain negative for the integrin. Three biological replicates per group were analyzed. C, Representative microscopic images of αvβ5 integrin (blue) expressed in one of the five human PDAC specimens analyzed. Red box, with cancer ducts; green box, without cancer ducts. Black arrowhead, cancer duct; white arrowhead, stroma cell. Scale bars, 500 μm (left); 50 μm (middle); 20 μm (right). Statistical analysis, Student’s t test. Error bars, mean ± standard error.

Article Snippet: The CD4 + T cells were cultured for 3 days with or without KPC78 cells in the presence of low dose anti-CD3/CD28 beads (x40 dilution; Cat# 130-095-925; Miltenyi Biotec), recombinant mouse TGF-β1 (5 ng/ml; Cat# 7666-MB; R&D Systems, Minneapolis, MN), and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Cat# 11271164001; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere.

Techniques: Flow Cytometry